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Barker SA, Borjigin J, Lomnicka I, Strassman R. 
“LC/MS/MS Analysis of the Endogenous Dimethyltryptamine Hallucinogens, Their Precursors, and Major Metabolites in Rat Pineal Gland Microdialysate”. 
Biomedical Chromatography. 2013.
We report a qualitative liquid chromatography-tandem mass spectrometry (LC/MS/MS) method for the simultaneous analysis of the three known N, N-dimethyltryptamine endogenous hallucinogens, their precursors and metabolites, as well as melatonin and its metabolic precursors. The method was characterized using artificial cerebrospinal fluid (aCSF) as the matrix and was subsequently applied to the analysis of rat brain pineal gland-aCSF microdialysate. The method describes the simultaneous analysis of 23 chemically diverse compounds plus a deuterated internal standard by direct injection, requiring no dilution or extraction of the samples. The results demonstrate that this is a simple, sensitive, specific, and direct approach to the qualitative analysis of these compounds in this matrix. The protocol also employs stringent MS confirmatory criteria for the detection and confirmation of the compounds examined, including exact mass measurements. The excellent limits of detection and broad scope make it a valuable research tool for examining the endogenous hallucinogen pathways in the central nervous system. We report here, for the first time, the presence of DMT in pineal gland microdialysate obtained from the rat.
Comments and Responses to this Article
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Jun 8, 2013 22:30
Cottonwood Research #

Rick Strassman's Cottonwood Research Foundation's Blog Entry announcing this study.
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May 23, 2013 20:31
First Direct Evidence of DMT in Mammalian Pineal Gland in Many Years #

This paper is the first to do direct work to detect DMT in the mammalian pineal gland in many years. Dr. Rick Strassman, one of the authors of this article, has long argued that DMT is probably produced in the pineal gland and this paper demonstrates to a strong level of certainty that DMT is produced in vivo in rat brains. Combining this with the previous evidence, this research suggests that DMT is also produced in the human pineal gland.

Very exciting!
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