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Nakahara Y, Kikura R, Takahashi K, Foltz RL, Mieczkowski T. 
“Detection of LSD and metabolite in rat hair and human hair”. 
J Anal Toxicol. 1996 Sep 11;20(5):323-9.
To examine the feasibility of detecting lysergic acid diethylamide LSD and its metabolites in hair, LSD was administered to rats with pigmented hair at 0.05, 0.1, 0.5, 1, and 2 mg/kg intraperitoneally once per day for 10 successive days. The rats were shaved just before the first administration, and newly grown hair was collected 4 weeks later. After being washed with 0.1 sodium dodecyl sulfonate and water and being dried in a desiccator, each 20-mg hair sample was extracted with 2 mliter methanol-5N HCl 20:1 under ultrasonication for 1 h and stored at room temperature for 14 h. The extract was evaporated to dryness, extracted from 0.1M NaOH with dichloromethane, and derivatized with a mixture of trimethylsilylimidazole, bis-trimethylsilylacetamide, and trimethylchlorosilane 3:3:2, v/v/v for gas chromatographic-mass spectrometric GC-MS analysis using LSD-d10 or lysergic acid methylpropylamide LAMPA as the internal standard. Selected ions were monitored at m/z 395, 293, and 279 for TMS-LSD and at m/z 381, 279, and 254 for the trimethylsilyl derivative of N-demethyl-LSD TMS-norLSD. LSD and norLSD were also detected by high-performance liquid chromatography HPLC with fluorometric detection excitation, 315 nm emission, 420 nm. LSD was detected in the rat hair following the lowest dose 0.05 mg/kg, whereas norLSD was only detectable in the hair following the highest dose 2 mg/kg. The same GC-MS and HPLC assays were applied to the analysis of hair from 17 self-reported LSD users, and LSD was detected in two of the samples.
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