Abramson HA, Evans LT, Geronimus LH, Haggis A, Jarvik ME, Kornetsky C, Wallace B, Wickersham CW.
“Bioassays for LSD-25”.
Annual Rep. Biol. Laborat.. 1954.
As the investigation of the psychodynamic effects of lysergic acid diethylamide (LSD-25) has progressed, it has become more and more evident that specific assay for minute amounts of this drug is necessary for the exploration of a number of avenues of research which present themselves. A concentrated effort was therefore made to find a bioassay for LSD-25. The biological rather than the chemical approach was chosen not only because it seemed more promising, but also because the search for a bioassay implies the investigation of the mode of action of the drug. In fact, certain portions of the program set up were perhaps more oriented toward an investigation the effect of LSD-25 than toward a bioassay for it. Such a primarily biological study was the following embryological one, which was based upon the rationale that, since LSD-25, after administration to adult animals, elicits manifestations which may be referable to an effect upon the central nervous system, it could be conjectured that this compound might have some ? effect on the early development of the nervous system of the chicken embryo. Experiments were therefore peformed in which fertile hen's eggs were injected at various times of incubation with varying amounts of LSD-25 and then excised after 8 days of incubation and examined grossly for visible abnormalities. Thus, distilled-water solutions containing 0.2, 0.5, 1.0, and ? mg of LSD-25 were injected into groups of eggs before incubation and 24, 48, 72, and 96 hours after the beginning of incubation. Non-injected eggs, as well as eggs injected with appropriate solutions of neutral ? tartrate (LSD.25 is actually d-lysergic acid diethylamide tartrate), as controls. It was found that there were no significant differences in frequencies or types of abnormalities observed in the embryos from non-injected, sodium tartrate injected, and LSD-25 injected eggs.
Various animals, both vertebrate and invertebrate, were treated with LSD-25 and observed for obvious vegetative, motor, or behavioral response,,. teat animals included crustacea, molluscs, amphibia, turtles, insects, and fish.
|Notes # : Cold Spring Harbor, New York 1954-1955
(auch BOL-, DHE-, Gy-, LAE-Erw.A)