Erowid References Database
Watt RM, Sissors DL, Voss EW.
“Molecular Weights Of Intracellular And Secreted Lysergyl Containing Peptides From Rabbit Lymphocytes And Murine Mopc-315 Myeloma Cells”.
In order to study the mode of action of drugs of mammalian protein biosynthesis, lysergyl containing peptides have been obtained from rabbit immune lymphocytes and murine plasma cytoma cells incubated in vitro in the presence of d-lysergic acid diethylamide (LSD). The peptides harvested bear covalent intrinsic radio-labels of 14C-LSD, 3H-LSD, or 3H-leucine. The molecular weights of both the intracellular and extracelluiar materials have been determined or estimated by gel filtration in denaturing solvents and SDS-polyacrylamide gel electrophoresis. A relatively large molecular weight component (> 55,000) and a lower molecular weight component (c 5,000) are the principal components quantitatively in both materials. The lysergyl peptides have also been analyzed in immuno-precipitin assays as to their structural relationship to the immunoglobulin molecule or other cellular proteins. Results suggest that LSD interferes with the de nova biosynthesis of several cellular proteins in the lymphocyte and is not specific to the immunoglobulin molecule.
|Notes # : Abstr.No. 2109
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