Erowid References Database
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Rucman R.
“Lysergsäure. II. Isolation und Trennung der Lysergsäuren”.
J.Chromatog.. 1976;121(2):353-60.
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Abstract
The isolation and separation of D-lysergic acid and D isolysergic acid is described. The raw materials used was waste from the industrial processing of ergot, containing 0.8% water-soluble alkaloids, 0.5 0.000000e+00rgotamine, 21.0 0.000000e+00rgosine, 20.8 0.000000e+00rgocornine, 12.7 0.000000e+00rgocryptine and 44.2 0extrorotatory alkaloids, with a total alkaloid content of 44.7%. Of various columns tested, best separation of this mix ture was obtained on columns filled with porous glass powder CMCG-10-75, using water containing 0.1% ammonia as eluant. Rotational data and TLC analysis (with fluorimetric quantitation) of.the lysergic acid fraction, which was the last to elute, showed it to be mainly D-lysergic acid containing 3-15 0solysergic acid. Although the presence of isolysergic acid normally does not interfere with further syntheses, separation of the 2 components as possible by further column chromatography on silica gel, using EtOH or-PrOH as eluant, the fractions thus obtained were almost pure lysergic acid containing 1.85 0solysergic acid and isolysergic acid containing 14.2% lysergic acid.
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