Erowid
 
 
Plants - Drugs Mind - Spirit Freedom - Law Arts - Culture Library  
Erowid References Database
Gershon MD, Robinson RG, Ross LL. 
“Serotonin Accumulation in the Guinea-Pig Myenteric Plexus: Ion Dependence, Structure-Activity Relationship and the Effect of Drugs”. 
J.Pharmacol.Exp.Ther.. 1976;198(3):548-561.
Abstract
The specific accumulation of serotonin (5-HT) in neurons of the guinea pig myenteric plexus was studied. Methods Male albino English short hair guinea pigs (300-350 g) were killed and the longitudinal muscle layer and adherent myenteric plexus (MP) were dissected and incubated in Kreb's solution at 37 under an atmosphere of 9502-5% CO2. The effects of 5-H 5-hydroxyindole, 5-hydroxy-N-methyltryptamine (5-HNMT) bufotenine, bufoteninium, tryptamine, 6-hydroxytryptamine (6-HT), 5,6,-dihydroxytryptamine (5,6-DHT), LSD, desmethylimipramin (DMI), imipramine (IMI), chlorimipramine (CMI), methamphetamine (MA) and p-chloro MA (PMA) on uptake of 3H-5-HT and 3H-noradrenaline (3H-NE) into the MP were studied. 3H-5-HT accumulation, known to be sodium dependent was inhibited by elevating external K levels. Accumulation was inversely related-to K concentration and was also decreased by or 12 mM Ca2+. The affinity 4f analogous molecules for the MP was greatly reduced by removal of the allcyl amino side chain, methylation of the terminal amino group or removal of the 5-OH group. Most analogs competitively inhibited 5-HT accumulatior and 6-HT was shown by histofluorescence to be taken up into th, MP after chemical sympathectomy with 6-hydroxydopamine. CMI was the most potent tricyclic antidepressant against 5-HT accumulation while DMI was the most potent inhibitor of NE accumulation. Amphetamines were more effective inhibitors of 5-HT accumulation than were tricyclic antidepressants, but all were more effective against NE than 5-HT accumulation. Conclusions The existence of a unique population of axons in the mammalian MP which accumulate 5-HT and that have not been found elsewhere in the peripheral nervous system is confirmed.
Comments and Responses to this Article
#
Submit Comment
[ Cite HTML ]