Erowid References Database
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Winkelhake JL.
“Effects of d-Lysergic Acid Diethylamide on Rabbit Antibody Biosynthesis in Vitro”.
Dissertation Abstr.Intern.B. 1975;35(7):3411-12.
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Abstract
The effect of LSD on rabbit antibody biosynthesis was studied in vitro. Rabbit anti-fluorescyl antibody biosynthesis was studied in vitro. Rabbit anti-fluorescyl antibody producing lymphoid cells incubated in vitro with LSD did not secrete 7S immunoglobulin. Secreted material was of low molecular weight and had no antibody activity. Cells incubated in vitro with LSD-(G)-3H released these peptides contained- a significarit portion of the lysergyl moiety covalently attached.'Isoelectric focusing indicated that cells cultured in the presence of LSD secreted discrete focusing components of pI 4.9 and 5.2. Molecular sieve chromatography and sodium dodecyl sulfate polyacrylamide gel electrophoresis showed the peptides to be smaller than 1-4,500 daltons. The lysergyl-peptides were soluble in 50% ammonium sulfate, thereby facilitating development of a radioimmune assay. Both immunochemical analysis utilizing ánti-lysergil antibody and chemical degradation indicated the lysergyl motety had undergone a miner modification on attachment to de-novo synthesized peptides. The lysergyl-peptides corresponded-to N-terminal regions of incomplete immunoglobulin-chains. The formation of the lysergyl-peptides could be inhibited with D- or L-tryptophan, some tryptophan analogs, puromycin, cyclohexamide and the non - hallucinogenic LSD analogue, d-lysergic acid(LSA). LSA was -10-fold less Efficient than LSD in causing hyperimmune cells to synthesize and secrete- lysergyl--peptides in vitro. Inhibition did not result with L-prolinej tyrosine, phenylalanine, histidine with glutamic acid or actinomycin-D. LSD bound to the 80S ribosome in a high affinity (E:'= 3.5'x 10 My) site. LSD Did not affect cell surface antigen binding, but capping ability was lost.
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