Erowid References Database
Voss EW, Cobb NC.
“Enzyme Capable of Modifying d-Lysergic Acid Diethylamide (LSD) in Rabbit Lymphoid Cells and Murine MOPC 315 Myeloma Cells”.
An enzyme capable of modifying LSD was identified in rabbit lymphoid cells and mouse MOPC 315 myeloma cells. Cell-free homogenates were prepared from antibody-producing - rabbit spleen and lymph node cells and from MOPC 315 tumor cells grown in BALB/c mice, and incubated with LSD-2-3H for 4-5 hr: the products were extracted in chloroform and separated by TLC. Lymphoid cell lysates from rabbits (including nonspecifically purified lymphocytes) catalysed the production of a hydrophilic product from 3H-LSD, and also incorporated LSD into low molecular weight peptides. MOPC 315 cells produced a similar hydrophilic product: the labeled metabolite was bound by anti-LSD antibody suggesting conservation of the 4-ring structure and staining with Ehrlich's reagent indicated absence of a 2'-substituent in ring B. The metabolite could replace tryptophan in a cell free protein synthesis system and may therefore be 'LSD-DER', a postulated LSD precursor. The enzyme was of high molecular weight and present in both the microsomes and cytosol; NAD, nicotinamide, oxygen and magnesium were required for activity
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