Erowid References Database
Gallager DW, Aghajanian GK.
“Chlorimipramine and LSD: Differential effects on the in vivo Release of (3)H-5HT”.
Certain biochemical and electrophysiological parameters do not distinguish between the effects of chlorimipramine (CIM) and LSD on the brain serotonergic (5HT) system: both drugs induce 5HT turnover and inhibit the firing of raphe (5HT) neurons. However, based on their proposed mechanisms of action, CIM (by blocking neuronal reuptake of 5HT) - should increase 5HT efflux and LSD (by directly inhibiting neuronal release of HIT) should decrease 5HT efflux. To examine these possibilities, the effects of these drugs on the in viva release of endogenous 3H-5HT (formed under 3H-L-tryptophan) were investigated via a ventricular push-pull cannula. Although CIM (5 mg/kg, i.p.) or LSD (100 mcg/kg. i.p.) both inhibited raphe cell firing for about 1 he j the efflux of 3H- 5HT v:as affected differenlally.by the 2 drugs
Chlorimipramine produced either an increase (20 mg/kg) or no change (5 mg/kg) in the efflux of 3H-5HT. However, LSD at both 100 and 200 mcg/kg produces a.decrease in 3H-5HT efflux.. These results demonstrated that although the effects of CIM and LSD on 5HT turnover and raphe neuronal firing were similar, distinct differences in their actions were revealed by measuring the efflux of 3H-5HT.
[ Cite HTML