Message-ID: <022309Z20081993@anon.penet.fi> Newsgroups: alt.drugs From: firstname.lastname@example.org (H-Man) Date: Fri, 20 Aug 1993 02:17:49 UTC Subject: VISINE to beat POT PISS-TESTS pt. 2 I posted a message the other day about using VISINE to cause false-negative POT PISS-TESTS. Well, I came down this morning and found that I was under a viaduct right near the university library, so I cleaned myself off a bit in nearby FOUNTAIN and ambled in for some further research. Here is a review of two articles concerning false-negatives through SPECIMEN alteration with EYE-DROPS and a bit of speculation about how to use this information. Please respond with COMMENTS or CRITICISMS. |--########>-- H-Man --<########--|  "Adulterants Causing False Negative in Illicit Drug Testing" Clinical Chemistry, Nov 1988, Volume 34, Number 11, p 2333-2336. Stephen L. Mikkelsen and K. Owen Ash  "Mechanism of False-Negative Urine Cannabinoid Immunoassay Screens by Visine Eyedrops" Clinical Chemistry, Apr 1989, Volume 35, Number 4, p. 636-638. Stephen D. Pearson, K. Owen Ash, and Francis M. Urry  describes the effects of 8 different urine-additives (table salt, Visine, handsoap, Drano, bleach, vinegar, goldenseal tea, and lemon juice) on the outcome of various drug tests. Every effective additive except Visine leaves an easily detected telltale sign: Drano, bleach and vinegar change the specimen's pH outside the normal range; goldenseal tea causes the specimen to turn brown; soap causes the specimen to become cloudy; and table salt forces the sample's relative density out of the normal range. Lemon juice did not affect the tests. The abstract notes: "Visine was the only adulterant not detected."  says that Visine does slightly reduce the urine sample's abaility to foam. Visine caused false negatives (EMIT d.a.u. assays) in the tests for benzodiazepines and marijuana, but did not change the test results for amphetamines, barbituates, cocaine, or opiates. 125 mL / L was the amount of Visine used per amount of urine specimen with 122 ug / L of 9-carboxy-THC in the sample.  focuses on the use of Visine to prevent pot metabolite detection. Here is a table summarizing the effectiveness of varying concentrations of Visine: Visine concentration (mL / L) : 0 10 20 50 100 200 300 Apparent 9-carboxy-THC concentration (ug / L) : 100 52 43 47 35 85 87 (Note the positive test cutoff value is 50 ug / L 9-carboxy-THC). The interesting thing to observe is the effectiveness of Visine as THC metabolite masker decreases as its concentration goes up past a certain point. The same pattern occured with the TDx and Abuscreen assays (the TDx assay generated false negatives, but the Abuscreen assay's apparent metabolite value fell only to 52 ug / L). The lesson to be learned is the optimum amount of Visine to use is around 100 mL / L. The ingredients of Visine responsible for the blocking of the THC test are given as benzalkonium chloride (a preservative) and the borate buffer. The mechanisms of the borate buffer were not discussed, but "reduced availability of 9-carboxy-THC in urine samples adulterated with Visine is due in part to uptake of 9-carboxy-THC into benzalkonium chloride micelles." To use this information to your advantage, figure that the standard urine specimen is 60 mL. At 100 mL / L Visine recommended, this means 6 mL of Visine would cut the observed 9-carboxy-THC almost two-thirds. Visine comes in 15 mL and 30 mL bottles. For advice on how to add the adulterant, read Abbie Hoffman's book on piss-testing, _Steal this Urine Test_. Use of other methods (such as diluting your urine by frequent water drinking) should be researched. Adding adulterants can be risky and should be seen as a last-ditch effort if you're caught with your pants down, so to speak. ------------------------------------------------------------------------- To find out more about the anon service, send mail to email@example.com. Due to the double-blind, any mail replies to this message will be anonymized, and an anonymous id will be allocated automatically. You have been warned. Please report any problems, inappropriate use etc. to firstname.lastname@example.org. ============================================================================= From: ACombs@utxvms.cc.utexas.edu (Alan B. Combs) Newsgroups: alt.drugs Subject: Re: VISINE to beat POT PISS-TESTS pt. 2 Date: 24 Aug 1993 02:37:04 GMT Message-ID:
> > The ingredients of Visine responsible for the blocking of the THC test are > given as benzalkonium chloride (a preservative) and the borate buffer. The > mechanisms of the borate buffer were not discussed, but "reduced > availability of 9-carboxy-THC in urine samples adulterated with Visine is > due in part to uptake of 9-carboxy-THC into benzalkonium chloride micelles." > If this is the mechanism and it becomes established, there probably would be a street market for Zephirin aqueous. It comes highly concentrated, and it is used as an antiseptic/surgical scrub, etc. The concentrate would have to be diluted appropriately. There are several less concentrated alcoholic sprays and tinctures. I suspect the alcohol would be quickly detected, however.