From: email@example.com Newsgroups: alt.drugs,alt.drugs.psychedelic Subject: +==SHROOMS: PF tips + Questions==+ Message-ID: <043303Z04041995@anon.penet.fi> Date: 6 Apr 95 17:06:14 GMT The following are tips and questions regarding the P.F. method. I will not reveal anything proprietary and I urge anyone interested to simply pay them their 10 bucks for their ideas. Please only reply if you are familiar with the PF method or are knowledgable about mycology. I have cultured a few jars in separate batches of four each. I have learned a little with each batch, which was the plan. I am just a couple of days short of the first projected "birthday." Tips: 1. I used both the low profile jars and the taller jars (all half-pint). Use the low profile jars/wide mouth. There is less glass space for the mycelium to cover, it seems. Also, somehow it seems to cover the bottom easier that way. 2. When mixing your substrate, add a little more water. But dont let the consistency get mud-like. By this I mean that when you load it in the jar, it fills in gaps without tapping it down much. This is bad. 3. When is says tamp down the substrate lightly when loading it in, they must really mean LIGHTLY. The most rapid growth has been on the jars that when you look at it through the glass, the substrate isnt pressed 100% up to the glass tightly. On the ones where I did pack it tightly, the mycelium started out aggressively, but ran into roadblocks. BTW, all substrate was mixed to death, I mean evenly. So I am thinking that it isnt a question of wet or dry clumps, but the loose/tight texture. Any comments? 4. Instead of an aquarium, you can go to Wal-Mart, The Container Store, Linens'n'Things, or whatever equivalent store near you, and buy a jumbo or extra-big clear plastic storage box (usually intended for blankets). I got mine for $9.99 and it has a snap-on lid and other nice features. You can burn a small hole in the bottom for drainage. 5. The clogging of the syringe was a problem for me. shake it alot and use a small stick pin to unclog it (flaming to sterilize). Questions: 1. For the jars where I suspect I have slowed growth due to substrate drying, how valid is this idea: Sterilize the syringes in the pressure cooker, and inject distilled water into the substrate? 2. For the jars where I have large areas uncolonized because no spores were injected (clogged needle, etc) when I did that side, could I re-inoculate after a week or two to speed things up? 3. For the jars where I have 100% coverage except for the bottom and 1/8 inch around from the bottom, could I give them a premature birthday, cut off the stubborn areas, and return them to the jars to await final coverage? (all done in draft free isolation). 4. THE MOST WORRISOME QUESTION: I have interfaced a inexpensive humidfier to the grow chamber (spin-motor, not ultrasonic). Will the air it blows in invite contamination? It has a small filter, but I suspect that it is not very effective. Does anyone have any experience with this? Also, is it appropriate to punch small holes for the air outflow? 5. Finally, what light and temp. are best to bring on the fruiting stage in the grow chamber? I have heard that temp. should be reduced from the incubation stage. Should there be further changes after fruiting begins for optimum rapid growth?