From: email@example.com (Peter Jordan) Newsgroups: alt.drugs Subject: Claviceps paspali references are HERE! Date: 29 Aug 1994 23:57:22 GMT Message-ID: <firstname.lastname@example.org> Interesting Chemical Abstracts References: CA: 71:P69351y : describes mutants of strain NRRL 3027 producing 2270 mg/l of ergoline compounds of which 85% were amides of lysergic and isolysergic acids. (Swiss patent application) CA: 77:P156333n : describes a method used to get 9.75g pure lysergamides from a 10 litre fermentation originally containing an estimated 15g. CA: 94:13992r : reference Indian Drugs; 1980; 17(8) 228-31 Eng. CA: 90:118108c : reference Indian Drugs; 1979; 16(4) 88-93 Eng. Very interesting>:-|| CA: 102:219574y : Serbo-Croat areticle describing 2.2g/l prod. by C. paspali Stevens and Hall (1984). Nature reference: Nature: 187:238-239. I can't remember this one but I have written down "-production by C. paspali in submerged culture" . Lloydia references: lots; just go check the indexes for each volume. An absolute Gem from 1916: Life History and Poisonous Properties of Claviceps paspali; H. B. Brown (Mississippi Agricultural Experiment Station); Journal of Agricultural Research ,vol. 7(9), pgs 401-405. describes on brief glance through it --- germination of the yellowish-grey sclerotia found on Paspalum dilatatum Poir. observed in the region of the Mississippi Agricultural College. Also contains reference to Stevens and Halls' original article (1910). "So there you have it gentlemen, what more evidence do we need" Yow!!! If you find this usefull/helpfull/interesting, let me know and I'll post a whole lot more. My willingness to post this stuff is directly correlated to the magnitude of the responses I get. Thanks very much for the encouragement from those who responded to the earlier postings. (Two from Germany and Two from USA). P.S. If you find a good culture of this stuff, I would LOVE to have some. P.P.S. If you take your rights, you'll never have them. ME! Peter Jordan ============================================================================= From: email@example.com (Peter Jordan) Newsgroups: alt.drugs Subject: More Claviceps references! Date: 31 Aug 1994 20:16:23 GMT Message-ID: <firstname.lastname@example.org> Hi again, Enjoy these goodies: * Biotechnological Exploitation of the Ergot Fungus (Claviceps Purpurea); Karl Esser and Andrea Duvell; Process Biochemistry, August 1984 pgs 142-149. Synopsis: "The alkaloids of the ergot fungus C. purpurea and related species already known as drugs in the middle ages are still finding many uses in medical therapy (he he). Since the supply of natural grown ergots is not sufficient, the biotechnological production of ergot alkaloids has gained in importance. This requires not only an undestanding of physiological and environmental conditions, but also concerted breeding in order to increase and stabilize the production level." * Biology of Claviceps; Willard A. Taber; Chapter 15 (sorry - i don't remember which book this came from, but it should be indexed under Taber in Biological Abstracts) pgs 449-486. "If one desires isolates of C. paspali (which are high producers of simple amides), one must go to paspalum grass. ... C. paspali differs from all other species in possesing a yellowish tan cauliflower- shaped sclerotium rather than the purplish banana-shaped sclerotium, and it has been suggested that this species be trasnsferred to the genus Mothesia. " *The Ergot Alkaloids; A. Stoll and A. Hofmann (THE); Chapter 21, The Alkaloids, Manske (ed. ?) vol. VIII, pgs 725-779+. Describes lots and lots and lots of chemical detail regarding everything from biogenesis to complete chemical synthesis as a means of confirming structure. Also has a section completely devoted to "Derivatives of Ergot Alkaloids" in which the following processes for synthesizing amides are discussed: => The azide process. => DMF-SO3 mixed anhydride method. => mixed Lysergic acid trifluoroacetic anhydride. => Lysergic acid chloride hydrochloride method. => N,N'- carbonyldiimidazole as condensing agent (Best IMHO). My fingers are tired, PLEASE do not rip any articles or pages from journals/books, as this is the most insidious kind of censorship (something a pig might do). Untill Later; Peter Jordan P.S. The P.P.S. of my previous posting should have read: "If you DON'T ........"