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Message-ID: <022309Z20081993@anon.penet.fi>
Newsgroups: alt.drugs
From: an13187@anon.penet.fi (H-Man)
Date: Fri, 20 Aug 1993 02:17:49 UTC
Subject:  VISINE to beat POT PISS-TESTS pt. 2

I posted a message the other day about using VISINE to cause false-negative
POT PISS-TESTS.  Well, I came down this morning and found that I was under a
viaduct right near the university library, so I cleaned myself off a bit in
nearby FOUNTAIN and ambled in for some further research.  Here is a review
of two articles concerning false-negatives through SPECIMEN alteration with 
EYE-DROPS and a bit of speculation about how to use this information.

Please respond with COMMENTS or CRITICISMS.

  |--########>--  H-Man  --<########--|

[1] "Adulterants Causing False Negative in Illicit Drug Testing"
    Clinical Chemistry, Nov 1988, Volume 34, Number 11, p 2333-2336.
    Stephen L. Mikkelsen and K. Owen Ash

[2] "Mechanism of False-Negative Urine Cannabinoid Immunoassay Screens by
    Visine Eyedrops"
    Clinical Chemistry, Apr 1989, Volume 35, Number 4, p. 636-638.
    Stephen D. Pearson, K. Owen Ash, and Francis M. Urry

[1] describes the effects of 8 different urine-additives (table salt,
Visine, handsoap, Drano, bleach, vinegar, goldenseal tea, and lemon juice)
on the outcome of various drug tests.  Every effective additive except
Visine leaves an easily detected telltale sign: Drano, bleach and vinegar 
change the specimen's pH outside the normal range; goldenseal tea causes
the specimen to turn brown; soap causes the specimen to become cloudy; and
table salt forces the sample's relative density out of the normal range.
Lemon juice did not affect the tests.  The abstract notes: "Visine was the
only adulterant not detected."  [2] says that Visine does slightly reduce
the urine sample's abaility to foam.

Visine caused false negatives (EMIT d.a.u. assays) in the tests for
benzodiazepines and marijuana, but did not change the test results for
amphetamines, barbituates, cocaine, or opiates.  125 mL / L was the amount
of Visine used per amount of urine specimen with 122 ug / L of
9-carboxy-THC in the sample.

[2] focuses on the use of Visine to prevent pot metabolite detection.  Here
is a table summarizing the effectiveness of varying concentrations of
Visine: 

Visine concentration (mL / L) :                 0   10  20  50  100 200 300
Apparent 9-carboxy-THC concentration (ug / L) : 100 52  43  47  35  85  87

(Note the positive test cutoff value is 50 ug / L 9-carboxy-THC).

The interesting thing to observe is the effectiveness of Visine as THC
metabolite masker decreases as its concentration goes up past a certain
point.  The same pattern occured with the TDx and Abuscreen assays (the TDx
assay generated false negatives, but the Abuscreen assay's apparent
metabolite value fell only to 52 ug / L).  The lesson to be learned is the
optimum amount of Visine to use is around 100 mL / L.

The ingredients of Visine responsible for the blocking of the THC test are
given as benzalkonium chloride (a preservative) and the borate buffer.  The
mechanisms of the borate buffer were not discussed, but "reduced
availability of 9-carboxy-THC in urine samples adulterated with Visine is
due in part to uptake of 9-carboxy-THC into benzalkonium chloride micelles."

To use this information to your advantage, figure that the standard urine
specimen is 60 mL.  At 100 mL / L Visine recommended, this means 6 mL of
Visine would cut the observed 9-carboxy-THC almost two-thirds.  Visine comes
in 15 mL and 30 mL bottles.  For advice on how to add the adulterant, read
Abbie Hoffman's book on piss-testing, _Steal this Urine Test_.  Use of other
methods (such as diluting your urine by frequent water drinking) should be
researched.  Adding adulterants can be risky and should be seen as a
last-ditch effort if you're caught with your pants down, so to speak.
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=============================================================================

From: ACombs@utxvms.cc.utexas.edu (Alan B. Combs)
Newsgroups: alt.drugs
Subject: Re: VISINE to beat POT PISS-TESTS pt. 2
Date: 24 Aug 1993 02:37:04 GMT
Message-ID: 

> 
> The ingredients of Visine responsible for the blocking of the THC test are
> given as benzalkonium chloride (a preservative) and the borate buffer.  The
> mechanisms of the borate buffer were not discussed, but "reduced
> availability of 9-carboxy-THC in urine samples adulterated with Visine is
> due in part to uptake of 9-carboxy-THC into benzalkonium chloride micelles."
> 

If this is the mechanism and it becomes established, there probably would
be a street market for Zephirin aqueous.  It comes highly concentrated, and
it is used as an antiseptic/surgical scrub, etc.  The concentrate would
have
to be diluted appropriately.  There are several less concentrated alcoholic
sprays and tinctures.  I suspect the alcohol would be quickly detected,
however.