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Oram 1970 found that increased temperatures resulted in higher DMT and total alkaloid levels in all ecotypes.

Moore et al. 1967 reported that the highest alkaloid levels (69.3 mg per 100 gram dry weight total alkaloid) were seen in plants experiencing 21^o C days, 16^o C nights. These plants also showed the greatest yield of plant weight (36.9 grams of dry weight per 18 plants.)