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Hochstein & Paradies 1957; obtained a mixture of Harmine, Tetrahydroharmine and Harmaline from previously brewed Ayahuasca.

They found that the entire mixture sublimed [Note] completely at 185° (0.1 mm).

They separated it using Florisil [an activated Magnesium silicate].

Chromatographing 0.5 grams of mixed alkaloids over 30 grams of Florisil.

Elution was with Chloroform followed by stepwise increments of Chloroform and methanol, ending with pure Methanol.

Harmine came off first, Tetrahydroharmine second and Harmaline last.

Ghosal & Mazumdar 1971 similarly reported they had eluted Harmine from alumina with Chloroform and eluted Harmaline with Methanol.